Layanan journal yang disediakan oleh Perpustakaan Universitas Gunadarma
| Judul Artikel | : | Hexane neem leaf extract more potent than ethanol extract against Aspergillus .flavus |
|---|---|---|
| Judul Terbitan | : | Universa Medicina |
| ISSN | : | 19073062 |
| Bahasa | : | ENG |
| Tempat Terbit | : | Jakarta |
| Tahun | : | 0000 |
| Volume | : | Vol. 33 Issue 3 0000 |
| Penerbit | : | Medical Faculty, Trisakti University |
| Frekuensi Penerbitan | : | 3 x 1 tahun |
| Penulis | : | Jenny Hidayat*, Ay Ly Margaret*, Hanna Yolanda**, and Lies K. Wibisono |
| Abstraksi | : | BACKGROUND Aspergillus jlavus is one of the causes of aspergillosis, with a high virulence and resistance to standard antifungals, resulting in a high mortality rate. Medicinal plants are increasingly used as they are relatively safer with minimal side effects. Previously we found that the ethanol extract ofneem (Azadirachta indica AJuss) leaves inhibits Aiflavus growth in vitro. However, most chemical compounds with antifungal effect are nonpolar. The purpose of this research was to compare the antifungal effect of neem leaves extracted in a nonpolar solvent to that of leaves extracted in a polar solvent. METHODS An in vitro experimental research was conducted between October 2013 and January 2014. Neem leaves were extracted in ethanol or hexane at various concentrations. A macrodilution test with 48-hour incubation time was done in triplicate on 8 groups of samples. These comprised the neem leaf ethanol extract (NLEE) at 0.5, 1.0, and 2.0 g/dl., neem leaf hexane extract (NLHE) at 0.5, 1.0, and 2.0 g/dL, positive control, and negative control groups. Fungal growth was detected on Sabouroud dextrose agar. Statistical analysis used Chi square and Fishers exact test. RESULTS NLHE had a higher, but statistically non-significant, inhibitory effect on A. flavus than NLEE (p=0.996). At higher concentrations, the antifungal effect ofNLHE is better than that ofNLEE. CONCLUSION There is no significant difference in in-vitro inhibitory effectivity on A. flavus ofneem leaves between extracts in polar and nonpolar solvents. |
| Kata Kunci | : | Azadirachta indica; neem; A. flavus; ethanol; hexane; antifungal |
| Lokasi | : | P171 |
| Terakreditasi | : | belum |