Layanan journal yang disediakan oleh Perpustakaan Universitas Gunadarma
| Judul Artikel | : | IFNy modulates human immunoglobulin receptor expression in lipoaspirate-derived mesenchymal stem cells |
|---|---|---|
| Judul Terbitan | : | Medical Journal of Indonesia |
| ISSN | : | 08531773 |
| Bahasa | : | ENG |
| Tempat Terbit | : | Jakarta |
| Tahun | : | 0000 |
| Volume | : | Vol. 23 Issue 3 0000 |
| Penerbit | : | Faculty of Medicine Universitas Indonesia |
| Frekuensi Penerbitan | : | - |
| Penulis | : | Dian R. Laksmitawati, Jeanne A. Pawitan.Mohamad Sadikin, Caroline T. Sardjono, Ahmad R. Utorno |
| Abstraksi | : | Background: Mesenchymal stem cell (MSC) has been reported to have immunomodulator capacity against autoimmune diseases and to prevent allogenic tissue rejection. Many studies revealed that MSCs inhibit T cell proliferation and induce immunosuppressive condition through the production of prostaglandins, and interieukin-IO. In addition, MSC was reported to reduce circulating autoantibody in autoimmune patients following MSC transfusion. So far, there has been no report stating the presence ofFc receptors (receptors for immunoglobulin) on MSCs. The aim of this study was to reveal the expression of FcyRs in lipoaspirate-derived MSCs by measuring transcription of FcyR mRNA and whether the expression can be modulated. Methods: Lipoaspirate-derived MSCs were cultured in suitable medium and confirmed to be MSCs according to the criteria published by International Society for Cellular Therapy. Total mRNA of MSCs was isolated, and detection of human FcyRI, FcyRIIA and FcyRIIB mRNA was performed. Further, modulation of the expression was tested using heat aggregated gamma globulin (HAGG) and interferon (IFN)y. Results: FcyRs mRNA was detected in the first passage of MSCs. However, the expression was no longer present after more than 4 passages. Further, increased level of FcyRI and FcyRlIA mRNA expression was detected with the addition of IFNy in the culture. This preliminary finding opens a new insight for the understanding of interaction between MSCs and immunoglobulin G through FcyRs. Conclusion: Lipoaspirate-derived MSCs express FcyRs, and the expression is modulated by IFNy. |
| Kata Kunci | : | Fcy receptor; interferon y; immunoglobulin G; mesenchymal stem cell |
| Lokasi | : | P127 |
| Terakreditasi | : | belum |