JOURNAL

Layanan journal yang disediakan oleh Perpustakaan Universitas Gunadarma

Metalloproteinase-9 gene variants and risk for hypertension among ethnic Javanese

Judul Artikel:Metalloproteinase-9 gene variants and risk for hypertension among ethnic Javanese
Judul Terbitan:Universa Medicina
ISSN:19073062
Bahasa:ENG
Tempat Terbit:Jakarta
Tahun:0000
Volume:Vol. 33 Issue 3 0000
Penerbit:Medical Faculty, Trisakti University
Frekuensi Penerbitan:3 x 1 tahun
Penulis:Fitranto Arjadi*, Saefuddin Aziz**, and Alfi Muntafiah
Abstraksi:BACKGROUND Hypertension is associated with endothelial-dependent vasodilation disorders, due to reduced nitric oxide (NO) availability and excessive angiotensin II (ANG- II) activation. The objective of this study was to determine the association between matrix metallopeptidase 9 (MMP-9) gene polymorphism and hypertension in ethnic Javanese in the 40-80 year age group. METHODS This was a case-control study on 50 PROLANIS patients of family doctors meeting the inclusion criteria and 50 controls without hypertension. Subjects were hypertensive patients with constant systolic arterial pressure of> 140 mmHg and diastolic arterial pressure of >90 mmHg, confirmed in three successive measurements The observed parameters were degree of MMP-9 polymorphism, and NO and ANG-TI levels. Matrix metallopeptidase 9 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using the Sma! restriction enzyme. MMP-9 polymorph isms were indicated by variation in band patterns. Degree of polymorphism in cases and controls were compared with NO and ANG-II levels in both groups. Data analysis was done using independent t-test, RESULTS The heterozygous (3 band) to normal (2 band) MMP-9 genotype ratio was 3: 1 in hypertensives, but balanced in controls. In hypertensives, heterozygous GA and homozygous AA genotype frequencies were respectively 3.198 and 1.548 times higher than that of the GG genotype (p=0.008 and p=0.726). There was a statistically significant differences of NO and Ang-II levels between cases and controls (p=O.OOO and p=O.OOO; respectively). CONCLUSION Matrix metallopeptidase 9 gene polymorph isms in hypertensive ethnic Javanese are associated with NO and angiotensin II levels.
Kata Kunci:Hypertension; ethnic Javanese; MMP-9 gene polymorphism; adults
Lokasi:P213
Terakreditasi:belum