Layanan journal yang disediakan oleh Perpustakaan Universitas Gunadarma
| Judul Artikel | : | Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau |
|---|---|---|
| Judul Terbitan | : | Universa Medicina |
| ISSN | : | 19073062 |
| Bahasa | : | ENG |
| Tempat Terbit | : | Jakarta |
| Tahun | : | 0000 |
| Volume | : | Vol. 33 Issue 3 0000 |
| Penerbit | : | Medical Faculty, Trisakti University |
| Frekuensi Penerbitan | : | 3 x 1 tahun |
| Penulis | : | Syamsurizal*, Yanwirasti**, Asman Manaf**, Jamsari***, ML Edy Parwanto****, and Arif Sardi |
| Abstraksi | : | BACKGROUND The prevalence oftype-2 diabetes mellitus (T2DM) has been increasing rapidly in developed and developing countries. Many new loci associated with T2DM have been uncovered by genetic and genome-wide association studies, ego the transcription factor 7-like 2 (TCF7L2) gene variant rs7895340 has been associated with type-2 diabetes mellitus. The aim of this research was to find an association between polymorph isms of TCF7L2 gene variant rs7895340 with T2DM in ethnic Minangkabau. METHODS This was a case-control study using a consecutive sampling technique among ethnic Minangkabau patients who came for treatment to the Metabolic Endocrinology Polyclinic at Dr. M. Jamil Hospital. Analysis was performed on 132 subjects (66 T2DM patients and 66 controls). DNA was isolated from venous blood samples and amplified using the amplification refractory mutation system - polymerase chain reaction (ARMS-PCR) method to detect single nucleotide polymorphisms (SNPs) of TCF7L2 gene variant rs7895340. To determine the significance of association ofSNPs ofrs7895340 with T2DM in ethnic Minangkabau, data analysis was performed using chi-square test. RESULTS The ARMS - PCR for TCF7L2 gene variant rs7895340 found polymorph isms in 28 (42.42%) subjects with type 2 diabetes mellitus and in 15 (22.72%) non- diabetic subjects.There was a significant association between rs7895340 SNPs with the probability ofT2DM in ethnic Minangkabau (OR=2.505, p=0.026). CONCLUSIONS Single nucleotide polymorphism rs7895340 of TCF7L2 gene can be used as genetic marker of type-2 diabetes mellitus in ethnic Minangkabau. |
| Kata Kunci | : | TCF7L2 gene; rs7895340 SNP; type-2 diabetes mellitus; ethnic Minangkabau |
| Lokasi | : | P205 |
| Terakreditasi | : | belum |